Jordaens never made the traditional trip to Italy to study classical and Renaissance art. Despite this, he made many efforts to study prints or works of Italian masters available in northern Europe. For example, Jordaens is known to have studied Titian , Veronese, Caravaggio, and Bassano, either through prints, copies or originals (such as Caravaggio's Madonna of the Rosary). His work, however, betrays local traditions, especially the genre traditions of Pieter Bruegel the Elder, in honestly depicting Flemish life with authenticity and showing common people in the act of celebratory expressions of life. His commissions frequently came from wealthy local Flemish patrons and clergy, although later in his career he worked for courts and governments across Europe. Besides a large output of monumental oil paintings he was a prolific tapestry designer, a career that reflects his early training as a "watercolor" painter.

Jordaens' importance can also be seen by his number of pupils; the Guild of St. Luke records fifteen official pupils from 1621 to 1667, but six others were recorded as pupils in court documents and not the Guild records, so it is probable that he had more students than officially recorded. Among them were his cousin and his son Jacob. Like Rubens and other artists at that time, Jordaens' studio relied on his assistants and pupils in the production of his paintings. Not many of these pupils went on to fame themselves, however a position in Jordaens's studio was highly desirable for young artists from across Europe.

Jordaens was greatly influenced by Peter Paul Rubens who occasionally employed him to reproduce small sketches in a larger format. After the death of Rubens, Jordaens advanced to the position of one of the most admired painters in Antwerp. Like Rubens, Jordaens relied on a warm palette, naturalism, and a mastery of chiaroscuro and tenebrism. Jordaens was only moderately successful as a portrait painter but excelled in representations of the base character of humanity. His classically inspired peasant themes and large scale Dutch moralistic genre scenes influenced Jan Steen. Jacob Jordaens did not specialize, however, he often repeated a theme based on a proverb that depicted a wide range of characters of a variety of ages, crowded in a festive scene around a banquet table. These humorous pieces have a sense of coarseness. While Jordaens drew upon Rubens' motifs throughout his career, his work is differentiated by a tendency to greater realism, a crowding of the surface of his compositions, and a preference for the burlesque, even within the context of religious and mythological subjects. Prometheus, c. 1640 is an example of the influence of both Rubens and Frans Snyders on Jacob Jordaens. While he drew inspiration from their collaboration Prometheus Bound, c. 1611-12, Jordaens' version is a more hopeful narrative.

In addition to being a well-known portrait painter, Jordaens also employed his pencil in biblical, mythological, and allegorical subjects and even etched a number of plates. Although primarily a history painter, he also painted illustrations of Flemish proverbs, such as the "Old Sing so the Young Twitter", and depictions of Flemish festivals, for example "The King Drinks". Several of his works hint at a passion for animal painting. It seems as if he often included a variety of animals, most likely drawn from life, including: cows, horses, poultry, cats, dogs, and sheep. His life drawings of both animals and people were used and referenced throughout his life. After Rubens, death in 1640 Jordeans became Antwerp's new leading artist. Only after achieving this status did Jordeans receive royal commissions, predominantly from the north. He also received a commission from Ruben's heirs to finish a Hercules and an Andromeda for Philip IV of Spain.

In 1635-40, when Rubens was ill from gout, Jordaens was commissioned to use Rubens' sketches, and work on the decorations for the triumphal entry of the Cardinal-Infante Ferdinand, the new Governor of the Spanish Netherlands, for his arrival in Antwerp in 1635. Although the works are lost, Jordaens was commissioned in 1639-40 by Charles I to finish decorating the Queen's chambers at Greenwich, a commission which was originally given to Rubens, who was unable to execute due to his poor health.

Jordaens also played his part in a collaborative effort to decorate the Torre de la Paroda, done between 1636 and 1681. Two works in the series attributed to Jordaens are Appollo and Pan (1637), made after a sketch by Rubens, and Vertummus and Pomona (1638). Further contributions debated include "Fall of the Titans", "Marriage of Peleus and Thitis", and "Cadmus Sawing the Dragons Teeth"(Vieghe, 262). In 1661, he was asked to paint three, fairly large lunettes for the newly constructed Amsterdam Town Hall.

The Protestant religion was forbidden in Antwerp, which at the time was still Spanish-occupied territory. Towards the end of his lifetime Jordaens converted to Protestantism, but continued to accept commissions to decorate Catholic churches. Jordaens was fined 200 pounds and 15 shillings for scandalous or heretical writings between 1651 and 1658. A monument was erected in Putte in 1877, dedicated to and containing the tombstones of Jordaens and two of his pupils, van Pape and Stalbemt. It stands on the location of the little Protestant church and cemetery, both of which were demolished years earlier. Jordaens died of the mysterious Antwerp disease ('zweetziekte' or 'polderkoorts' in Dutch) in October 1678, which, on the same day, also killed his unmarried daughter Elizabeth, who had lived with him. Their bodies were buried together under one tombstone in the Protestant cemetery at Putte, a village just north of the Dutch border, where his wife Catharina had been put to rest earlier. One year after his death, Jacob Jordaens' son donated "twenty-five Flemish pounds to the Camer van den Huysarmen in Antwerp." Also included in this donation was The Washing and Anointing of the Body of Christ which was given to an orphanage of girls. Apparently this was all done in following correspondence with a will that Jacob Jordaens left behind. Unfortunately, this document has yet to be found. Even without the finding of Jordaen's will, his kindness has been recognized by all who knew him. There are many other found documents that note his admiration by others.

At the end of Jordeans career between 1652-78 his creative and artistic ability had deteriorated. He moved from vibrant colors to a gray-blue palette, accented at times with a dull brown, and applied paint so thinly that the canvas could be seen. However, there were few exceptions to this (such as the aforementioned religious paintings he produced after he had converted to Protestantism), most notable being the History of the Psyche that he did for his own house.