He spent four years in the Florence region, absorbing the local art and different styles. In that period, he was exposed to Leonardo Da Vinci's works and techniques, learning from it and being highly influenced by him and his religious art. Michelangelo also had great importance to his development as an architect and painter, serving as inspiration and sharpening his skill. In 1501, Raphael was acknowledged as one of the great masters of the High Renaissance.

His last artistic phase was in Rome. In 1504, he was invited by the Popes Julius the Second and Leo the Tenth to move to the capital and work in commissions. He worked in some of the most fabulous masterpieces that were ever made, like the Stanza della Segnatura, The Hill of Calvary, The Cardinal Virtues, and The Sistine Madonna.

La Disputa was the first room that Sanzio painted in Vaticano. It is part of the Raphael Rooms and is his more detailed and rich theological work. Located at the Apostolic Palace, this formidable fresco displays multiple biblical figures and theologians discussing the Transubstantiation, with Christ's body represented by the Eucharist.

Raphael made a formidable work designing the fresco. The painting guides the viewer through the scene to the altar, located below the semi-circle of clouds where Jesus is sitting. The artist also made another hidden symbology in the painting. The horizontal line of clouds, where saints, prophets, and theologists sit, form a great cross with the Eucharist, God, Jesus Christ, and the Holy Spirit.

The Christ Glorified is the central figure of the fresco. Above him, God is painted with a wise and caring expression. At his left side sits the Virgin Mary, drawn with blue silk, that represents the church, and red dress, representing the death of her beloved son. At his right, John the Baptist, holding the Holy Cross. Jesus, emitting an aura of light, is shown sitting in a throne of clouds, surrounded by winged puttos. Below Christ, we can see cherubins holding books, representing his words and commandments.